 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : A Simple Method to Extract Mitochondrial DNA in a Non-invasive Phylogenetic Study of Domestic Native Thai Ducks) ผู้เขียน: ปิยะนันท์ ลีแก้ว,  ดร.ทวีศักดิ์ ส่งเสริม, ศาสตราจารย์ , ดร.อาภัสสรา ชูเทศะ, รองศาสตราจารย์ , ดร.อุคเดช บุญประกอบ, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractA simple low-cost method for extracting mitochondrial DNA (mtDNA) from a single plucked
feather for a non-invasive genetic study of ducks is described. The mtDNA was isolated via alkaline
lysis, and after neutralization, the crude DNA-containing lysate was used directly for PCR. We have had
a 100% (30/30) success in amplification of a 710-bp fragment of the mtDNA control D-loop region.
Sequence analysis and phylogenetic relationship of the 667-bp D-loop segments of the two domestic
native Thai ducks, Nakorn-Pathom (NP) and Park-Nam (PN), was further investigated. Two breeds
exhibited 100% identity over the entire 667-bp products, although several types of mtDNA polymorphism
were detected. Comparing the 667-bp D-loop sequence data with those of Anas ducks and Muscovy
duck used as an outgroup showed that both native Thai ducks and Mallard (Anas platyrhynchos) haplotype
A clustered together (bootstrap support 91%), indicating that there was common ancestor for those
breeds. Here we report a quick, simple, reliable and inexpensive DNA extraction procedure for a duck’s
feather. Our findings clearly demonstrate that the domestic native Thai ducks derive their origin from
the Mallard group A haplotypes. |
 |
|